| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1761 | Leptospira biflexa | AACCTGCCATTGTGATCGG | CGATCGCGTATGCATCTGC | 58.13 | 59.59 | 186 | 74 |
100.00%
|
100.00%
|
| Primer 1762 | Leptospira biflexa | TGGCAGCGTTTGGGTTTG | TTCACTTCGCCAGACCGAC | 59.19 | 60.01 | 193 | 74 |
100.00%
|
100.00%
|
| Primer 1763 | Leptospira biflexa | AGCCAACCTTTCCAAACTCC | ACTCCGCAATCCCTTTCGG | 58.30 | 60.08 | 197 | 74 |
100.00%
|
100.00%
|
| Primer 1764 | Leptospira biflexa | TGGGGTGAACGAGCCTTTC | ACCAATCGCACCACCCAA | 59.93 | 59.48 | 291 | 74 |
100.00%
|
100.00%
|
| Primer 1765 | Leptospira biflexa | CCGCATTCCTTGGTGGGAT | ACCAATCGCACCACCCAA | 60.08 | 59.48 | 254 | 74 |
100.00%
|
100.00%
|
| Primer 1766 | Leptospira biflexa | TGGGCAAGGTTACGATCGG | TGCACCCACAAACGACCA | 59.78 | 59.73 | 278 | 74 |
100.00%
|
100.00%
|
| Primer 1767 | Leptospira biflexa | TCCCAGGGACTTCTGCCAA | AGCCCAGCGACAATTGCA | 60.46 | 60.28 | 298 | 74 |
100.00%
|
100.00%
|
| Primer 1768 | Leptospira biflexa | AAACCCTGCCCTTGCGAT | ACGGGTTGTGCACACCAA | 59.56 | 60.04 | 216 | 74 |
100.00%
|
100.00%
|
| Primer 1769 | Leptospira biflexa | AAAACCCTGCCCTTGCGAT | ACGGGTTGTGCACACCAA | 60.23 | 60.04 | 217 | 74 |
100.00%
|
100.00%
|
| Primer 1770 | Leptospira biflexa | AGCAGCAGCCCAATCCAA | TTTCTGTCCGAAGCACCCC | 59.56 | 59.93 | 254 | 74 |
100.00%
|
100.00%
|